Srebp-1/2 (AA 301-450) anticorps

N° du produit ABIN686032

Cet anticorps Lapin Polyclonal détecte spécifiquement dans WB, IF (cc), IF (p), ELISA, IHC (fro) et IHC (p). Il présente une réactivité envers Boeuf (Vache), Humain, Souris et Rat et a été mentionné dans 2+ publications.

Aperçu rapide pour Srebp-1/2 (AA 301-450) anticorps (ABIN686032)

Antigène: Srebp-1/2

Reactivité: Boeuf (Vache), Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Épitope: AA 301-450

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Homologie: Sheep,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human SREBP-1

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Référence

Fu, Cui, Zhang: "Lack of ClC-2 Alleviates High Fat Diet-Induced Insulin Resistance and Non-Alcoholic Fatty Liver Disease." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 45, Issue 6, pp. 2187-2198, (2018) (PubMed).

Zhang, Zhang, Chen, Hu, Wang, Jin, Zhang, Wang, Wang, Kang, Li, Li, Pan, Huang, Kong: "Reactive Oxygen Species-Induced TXNIP Drives Fructose-Mediated Hepatic Inflammation and Lipid Accumulation Through NLRP3 Inflammasome Activation." dans: Antioxidants & redox signaling, Vol. 22, Issue 10, pp. 848-70, (2015) (PubMed).

Détail du antigène

Antigène: Srebp-1/2

Sujet:

Synonyms: Sterol regulatory element binding protein 1, ADD 1, BHLHD1, D630008H06, SREBF 1, SREBF1, SREBP 1, SREBP1, SREBP 1c, Sterol regulatory element binding protein 1, Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1, Sterol regulatory element binding transcription factor 1.

Background: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. [SUBUNIT] Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. [SUBCELLULAR LOCATION] Endoplasmic reticulum membrane, Multi-pass membrane protein. Golgi apparatus membrane, Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane, Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. [SUBCELLULAR LOCATION] Processed sterol regulatory element-binding protein 1: Nucleus. Belongs to the SREBP family.

ID gène: 6720, 6721